biological activity assay kit / for medical research / for enzymes / ATP
ADP-Glo™ Kinase Assay

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Characteristics

  • Applications:

    for biological activity, for medical research

  • Tested parameter:

    for enzymes, ATP

Description

One Assay for all your Kinase Screening Needs
Use with virtually any kinase
Stable luminescent signal
Sensitive to low concentrations of ADP
A luminescent ADP detection system for kinases and other ADP-generating enzymes
The ADP-Glo™ Kinase Assay measures ADP formed from a kinase reaction. The ADP is converted into ATP, which is used to generate light in a luciferase reaction. The luminescence generated correlates with kinase activity.

The assay is well suited for measuring the effects of chemical compounds on the activity of many purified kinases, making it ideal for primary screening as well as kinase selectivity profiling. Z'-Factors >0.7 are routinely obtained. The stable luminescent signal allows batch plate processing without the need for strictly timed incubations.