Serum reagent kit Triglyceride-Glo™

tissuephosphatelipase

Works with lysed cells, tissues, medium and serum samples No organic extraction, extreme heat or centrifugation required Linear triglyceride detection up to 80µM Rapid and Sensitive Triglyceride Detection in Biological Samples The Triglyceride-Glo™ Assay is a bioluminescent assay for rapid and sensitive measurement of triglycerides in cultured cell lysates and other biological samples, such as cell culture medium, serum and tissue homogenates. The assay is ideal for measuring triglyceride accumulation and clearance in normal and pathological conditions. Examples include adipocytes, liver samples or cell culture liver models where excess triglyceride accumulation causes steatosis, an early hallmark of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). How the Assay Works The Triglyceride-Glo™ Assay detects triglyceride levels by measuring glycerol that is released from an enzymatic reaction with a lipase: one mole of glycerol per mole of triglyceride. Glycerol is measured in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. The amount of triglyceride is determined from the difference of glycerol measured in the absence (free glycerol) and presence (total glycerol) of lipase. Lipase converts triglyceride (TAG) to glycerol. Glycerol kinase and glycerol-3-phosphate dehydrogenase are used to generate NADH. In the presence of NADH, Reductase enzymatically reduces a proluciferin Reductase Substrate to luciferin. Luciferin is detected in a luciferase reaction using Ultra-Glo™ Luciferase and ATP,