Stain reagent kit α-NAE

for hematology

This kit is for staining bone marrow cell and bloodcell smear. Principle: Hydrochloric acid parafuchsin reacts with Sodium Nitrite to form diazonium salt (six-azo-pararosaniline). α-Naphthyl Acetate is decomposed by esterase and produces α-naphthol, which shall combine with diazonium salt to form red brown precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called "Non-specific Esterase Stain". Methods: Working solution Preparation (for 1 test only): Apparatus required: Disposable tube, micropipette, disposable tips, dropper; Instruction: Add 50µl solution B and solution C in a test tube ; mix well and wait for 1 minute. Then, add 1.5ml solution D and 50µl solution E; mix well and wait for 2 minutes. (Add 1 drop of solution NaF for NaF inhibition test.) Expected Results: Red or brown granules found in cytoplasm are positive. ①Spot pattern: It is mainly seen in mature T Lymphocytes. 1~4 brown or red-brown circular, mass, and big spot granules with clear contour in cytoplasm are found in positive reaction. ②Diffuse pattern: Red-brown and dusty granules diffusely spread are found in positive reaction. Granules may appear at a certain part of cell with obscure outline in cytoplasm. ③Monocyte Pattern: Evenly stained red-brown granules diffusely spread in cytoplasm are found in positive reaction.