Stain reagent kit AS–DCE

for hematology

This kit is for staining bone marrow cell and bloodcell smear. Principle: Naphthol AS-D chloroacetate can be hydrolyzed into Naphthol AS-D by Esterase to mix with diazonium salt and form insoluble red-brown precipitate in cytoplasm. Positive reaction is only found in granulocytes; hence, it is also called "Specific Esterase Stain". Methods: Working solution Preparation（for 1 test only）; Apparatus required: disposable tube, micropipette, disposable tips, dropper; Instruction: Mix 10µl solution B and10µl solution C thoroughly. Wait for 1 Minute; Add 0.9ml solution D and 50µl solution E. Mix well; Expected Results: Red granules found in cytoplasm means positive.