- Laboratory
- Laboratory medicine
- Stain reagent
- Wuhan Jinhong Biotech Development Co., Ltd.
Stain reagent kit for hematologyalkaline phosphatase
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Characteristics
- Type
- stain
- Applications
- for hematology
- Tested parameter
- alkaline phosphatase
Description
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm.
Methods:
Working solution Preparation (for 1 test only)
Apparatus required：disposable tube, micropipette, disposable tips, dropper;
Instruction：Mix 25µl solution B with 25µl C; wait for 2 minutes. Add distilled water 2ml into 25µl solution D. Mix thoroughly.
Expected Results:
NΑP positive granules are blue.
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Marrow Cell Stains
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