Stain reagent kit

for hematology

This kit is for staining bone marrow cell and bloodcell smear. Principle: Under alkaline condition, α-Naphthyl Butyrate is hydrolyzed into α-naphthol by Esterase. α-naphthol shall combine with diazonium salt to forminsoluble colored precipitate in cytoplasm. This stain has no specificity against esterase; therefore, it is called"Non-specific Esterase Stain". Methods: Working solution Preparation (for 1 test only): Apparatus required: disposable tube, micropipette, disposable tips, dropper; Instruction: Add 10µl solution B to 10µl solution C, mix well and wait for 1 minute; Add 4ml solution D and 200µl solution E. Mix well (Add 2 drops of solution NaFfor NaF inhibition test.) Expected Results: Red or red-brown granules found in cytoplasm means positive.