Stain reagent kit PAS

for hematology

Intended Use: This kit is for staining bone marrow and blood cellsmear. Principle: PAS Stain is based on the principle of periodic acid-Schiff reaction. Periodic acid can oxidize Ethylene Glycol (-CHOH-CHOH) contained in glycogen polysaccharides of cells to turn into dialdehyde (-CHO-CHO). Subsequently, aldehyde will react with colorless Fucshin to form a red-fuchsin compound incytoplasm. Methods: 1. Dry the smear. Fix smear in solution A for 30 seconds and rinse with distilled water. Air dry or dry with absorbent paper. 2. Drop solution B on slide to fully cover the smear for 5minutes. Rinse with distilled water and dry with filter paper or dry in the air. 3. Drop solution C on slide to fully cover the smear for 10~15 minutes. Rinse with distilled water for 5 minutes. 4. Counterstain with solution D for 1~2 minutes. Rinsewith distilled water. Dry the smear for microscopic examination.