Stain reagent kit ACP

This kit is for staining bone marrow cell and bloodcell smear. Principle: Acid Phosphatese stain is based on diazotization-coupling principle. At pH0.5, Acid Phosphatase hydrolyzes Naphthol AS-Bl Phosphoric Acid and releases Naphthol AS-B1 to mix with diazonium salt and form insolublecolored precipitate in cytoplasm. Methods: Working Solution Preparation (for 1 test only) Apparatus required: disposable tube, micropipette, disposable tips, dropper Instruction: Mix 25µl solution B with 25µl solution C; wait for 2 minutes. Add distilled water 2ml into 25µl solution D and 90µl solution E. Mix thoroughly. Procedure: 1. Dry the smear and add solution A to fix the smear for 30 seconds. Rinse the smear with distilled water and dry it. 2. Add working solution and incubate at 37℃for 60 minutes. Rinse with distilled water and dry it. 3. Counterstain with solution F for 1~2 minutes. Rinsewith distilled water. Dry the smear for microscopic examination.