Stain reagent Sudan Black B Stain (SBB)

for hematologyfor cytologychromogenic

Intended Use: This kit is for staining bone marrow and blood cell smear. Principle: Sudan Black B is a fat-soluble stain used for staining triglycerides and lipids. In cytoplasm, Sudan Black B can stain granules dark brown. Specifications: - Contents: 3 vials ×100ml/Kit - Components: - Fixative: 1 vial ×100ml (Methanal) - Sudan Black B Solution: 1 vial ×100ml (Sudan Black B) - Wright Stain Solution: 1 vial ×100ml (Wright) Methods: - SBB stain is designed for dip method. - Fix dried bone marrow and blood smear in Fixative for 10~20 seconds. Rinse with distilled water. Air dry the smear. - Dip the smear into Sudan Black B solution and incubate at 37°C for 20~30 minutes. Rinse with distilled water; air dry or dry with filter paper. - Counterstain with Wright Stain. (Stain the smear with Wright Stain and phosphate buffer solution (1:2) for 5~10 minutes. Rinse with distilled water. Dry the smear for microscopic examination.) Expected Results: Dark brown granules found in cytoplasm means positive. - Product Name: Sudan Black B Stain (SBB) - Application: Staining of bone marrow and blood cell smears - Principle: Fat-soluble stain for triglycerides and lipids - Kit Contents: 3 vials ×100ml (Fixative, Sudan Black B Solution, Wright Stain Solution) - Components: Methanal, Sudan Black B, Wright - Method: Dip method, counterstain with Wright Stain - Expected Result: Dark brown granules in cytoplasm indicate positive