Stain reagent kit Papanicolaou Stain

for cytologyclinical

It is intended to stain and check cellular morphology in clinic. During gynecological examination, Papanicolaou stain is a commonly used screening method for cervical cancer and pathological changes prior to cancer. With Papanicolaou stain, the hormonal level of woman can be observed and any infection by pathogens in genital meatus such as Candidiasis and Trichomonas can also be detected. This Papanicolaou stain kit utilizing EA36 and EA50 is a modification of EA36 and both methods are essentially the same. Generally, EA36 and EA50 are mainly intended for gynecological specimen while other modified EA stains are intended for non-gynecological specimens (i.e. exfoliative cells from chest and ascites). Principle: Papanicolaou stain contains Hematoxylin, Orange G, Eosin, Bismark Brown and Light Green, etc. Oxidative Hematoxylin will bind nucleic acids of karyon and appear indigo. Other stains will bind different chemical components of cytoplasm and appear in different colors. Cytoplasmic dyes are prepared with high concentration alcohol and strict measures of hydrating and dehydrating are taken during staining, hence various components of the cell will bind the dyes well. In general, the structure of nucleolus is clear, cytoplasm is bright and vivid, and the granules in cytoplasm are evident. Therefore, Papanicolaou stain is a very useful cytology staining method. Methods: 1. Fix smears in 95% ethanol for more than 15 minutes. 2. Dip smears in 80% and 50% ethanol for 30 seconds each. Rinse with water gently. 3. Stain for 3~5 minutes with Harris. 4. Rinse with water for 1~2 minutes. 5. Differentiate in 0.5%~1% hydrochloric acid ethanol for several seconds.