Stain reagent kit BA4095

for histology

Mature hematin reacts with tungsten to form blue sediment which strongly combines with tissue. Reddish brown portion is stained by Phosphotungstic Acid. Methods: 1. Put tissue into xylene. In subsequent step, put the tissue in tap water. 2. Add distilled water into 5% iron alum and mordant for 30 minutes. 3. Rinse with distilled water after washing in tap water. 4. Oxidize the smear in 2.5% potassium permanganate and rinse with tap water. 5. Place in 2.5% oxalate till the smear turns white. 6. Rinse in tap water, distilled water. Then, dry with absorbent paper. 7. Place in PTAH stain for 12~24hours, or incubate for an hour. 8. Differentiate in 95% alcohol for 3~5 seconds.(As soon as possible) Rapidly dehydrate; clear and mount. Expected Results: Rhabdium, Glia, Fibrin, Nuclei……..……..Blue Collagen fibers……………………………..Reddish Brown Precaution: 1. Do not rinse with water after staining with Phosphotungstic Acid Haematoxylin. Quickly rinse in 95% alcohol, because alcohol or water will wash red color away. 2. Do not overstain the smear, since Phosphotungstic Acid Haematoxylin is progressive stainning reagent. Examine under a microscope in certain period. 3. The procedure of staining is to be operated in a staining jar.