Stain reagent BA4120A

for histology

Principle: O-sulfate esters are stained brown-black with HID generated “in-situ” by the oxidation of a mixture of hydrochlorides of N, N-dimethyl meta- and para-phenylene diamines with ferric chloride. In subsequent steps, sialic acid and carboxyl groups are stained aquamarine blue with Alcian Blue at pH 2.5. Specifications: - Contents: 25 Tests - Components: - Alcian blue solution (pH2.5): 1 vial × 20ml - HID-solution A: 1 vial × 100ml - HID-solution B: 1 vial × 20ml - Ferric Chloride solution: 1 vial × 10ml Working Solution Preparation: - HID-solution A: 40ml (20ml) - HID-solution B: 10ml (5ml) - Ferric Chloride: 1.5ml (0.75ml) - Prepare the solution prior to use. Methods: 1. Deparaffinize tissue in deionized water. 2. Place in working solution at room temperature for 18~24 hours. 3. Rinse in running tap water. 4. Stain in Alcian blue solution (pH2.5) for 10~20 minutes. 5. Briefly wash with water. (If necessary, counterstain nuclei to fix the red color.) 6. Dehydrate, clear and mount with mounting media by following routine procedure. Expected Results: - Acid Sulfmucus: Purple to Dark brown - Carboxylated Acid Mucus: Blue - Nuclei: Red (Counterstain nuclei to fix the red color.) Precaution: - Prepare high iron diamine solution prior to use, and discard after use. The solution can be used once or twice if well stored in a refrigerator. However, the background and specificity may be influenced and changed. - Place smear in working solution at room temperature between 20~25°C. At lower room temperature extend staining time. - Diamine salt is toxic. Avoid contact with skin. Technical Specifications / Features: - Product type: Histology Stain Kit - Test quantity: 25 tests - Components: Alcian blue solution, HID-solution A, HID-solution B, Ferric Chloride solution - Application: Differentiation of O-sulfate esters, sialic acid, and carboxyl groups in tissue samples - Storage: HID solution should be prepared fresh and can be stored in refrigerator for short term - Safety: Diamine salt is toxic, avoid skin contact