Stain reagent kit BA4086A

for histologyCryptococcus neoformans

Mucicarmine stain is very useful in the detection of epidermic mucus, and can also be used to stain the capsule of Cryptococcus neoformans. Principle: Carmine, also called Coccinellin, can bind mordant aluminum to form a complex, which in turn binds to the acid group of mucus and becomes red. The capsule of Cryptococcus neoformans consists of mucous substance and can be stained red with carmine. Methods: 1. Deparaffinize tissue in typical manner in deionized water. 2. Place in Gill Hematoxylin Solution for 10 minutes and rinse briefly with running water. 3. Blue in Scotts tap water Substitute for 1 minute and rinse briefly with running water. 4. Place in Mucicarmine stain for 20~30 minutes. 5. Rinse with running water for 2 minutes. 6. Counterstain in Tartrazine solution for 1~3 minutes after blotting up with filter paper. 7. Rinse briefly with distilled water. 8. Dehydrate in typical manner and clear. Mount with mounting media. Precaution: 1. Store Mucicamine stain at 4℃. 2. Do not use Ehrlich Hematoxylin to stain nuclei because it stains mucus lightly and interfers with carmine staining. 3. Do not counterstain Tartrazine solution excessively, or else the red of mucus will be masked. Counterstain is optional and can be omitted.