Sexually transmitted disease assay kit MOLgen

for DNATreponema pallidumtissue

“MOLgen DNA Treponema pallidum S1 Kit” is an assay kit for the detection of Treponema pallidumDNA by real-time PCR. Introduction “MOLgen DNA Treponema pallidum S1 Kit” is intended for the detection of Treponema pallidum DNA, in clinical specimens (swabs of the epithelial cells, tissue fluid of erosive–ulcerative skin lesion and mucosa), using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product. The extraction of DNA can be performed using the “Molgen Universal Extraction Kit”. When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis S.r.l. Set 1 is intended for use with block-type PCR cyclers:AMPLIlab (Adaltis), iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 48 tests, including control samples. NOTE: The extraction protocol and the PCR set-up procedure can be done in automated mode using the Adaltis EXTRAlab Instrument by executing the extraction and PCR set-up protocol preloaded in the instrument user interface. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.