Molecular diagnostic assay kit MOLgen

bloodserumplasma

“MOLgen RNA TBEV S1 Kit” is an assay kit for tick-borne encephalitis virus RNA detection by real-time PCR. Introduction “MOLgen RNA TBEV S1 Kit”is intended for the detection of the tick-borne encephalitis virus (TBE) RNA using reverse transcription of viral RNA and real-time polymerase chain reaction (RT-PCR) method with fluorescence detection of amplified product. The extraction of RNA from clinical specimens (whole blood, serum (plasma), leukocyte blood fraction, cerebrospinal fluid, biopsy materials and tick suspensions) can be performed using “Molgen Universal Extraction Kit”. When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis. The results of PCR analysis are taken into account in complex diagnostics of disease. Set 1 is validated for use with block-type PCR cyclers: iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia). Set 1 contains reagents requiredfor 48 tests, including control samples. Principle Of Method Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.