Pneumonia test kit MOLgen

COVID-19coronavirusSARS-COV-2

MOLgen SARS-CoV-2 Real Time RT-PCR Kit is a multiplex one-step RT-PCR assay for the qualitative detection of Novel Coronavirus (SARS-CoV-2), by Reverse Transcription (RT) and Real Time Polymerase Chain Reaction (PCR) from RNA extracted from human respiratory specimens such as nasopharyngeal swabs, oropharyngeal swabs, salivary specimens and bronchoalveolar lavage fluid (BALF). Introduction Coronaviruses are a large family of respiratory viruses, some causing illness in human and others circulating among animals such as camels, cats and bats from common cold to severe diseases such as Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a new strain of coronavirus emerged from Wuhan, China in December 2019. The infection with SARS-CoV-2 virus causes Coronavirus disease (COVID-19) where "CO" stands for crown, "VI" for viruses, "D" for disease and “19'" indicates the year in which it occurred. COVID-19 symptoms are fever, cough, pneumonia and Severe Acute Respiratory Syndrome (SARS). Principle Of Method MOLgen SARS-CoV-2 Real Time RT-PCR Kit is a multiplex real-time one-step RT-PCR assay for the detection of the New Coronavirus SARS-CoV-2 RNA using reverse transcription of viral RNA and real-time polymerase chain reaction (RT-PCR) method with fluorescence detection of amplified product. The primer and probe set is designed to detect 4 targets for SARS-CoV-2: 3 specific for SARS-CoV-2 (N gene, S Gene, E gene) and 1 common for sabercoviridae (RdRp gene). The detection of amplified virus RNA fragments is performed in fluorimeter channels: FAM (RdRp gene),