Molecular diagnostic assay kit ME184730

HPVurineclinical

“Molgen DNA HPV 16/18 S1 Kit” is an assay kit for the detection of Papillomavirus Type 16 and 18 DNA by Real-Time PCR method. Introduction “Molgen DNA HPV 16/18 S1 Kit” is designed to detect DNA of human papillomavirus type 16 and 18 isolated from clinical specimens using extraction kit: “MOLgen Universal Extraction Kit”. Assay is based on real-time polymerase chain reaction (PCR) method with fluorescent detection of amplified product. The kit contains reagents requiredfor 96 tests, including control samples. The kit is validated for use with iQ5 iCycler (Bio-Rad, USA). The kit is also compatible with CFX96 (Bio-Rad, USA) and DT-96 (DNA-Technology, Russia) PCR systems. “Molgen DNA HPV 16/18 S1 Kit” is designed for the analysis of clinical materials (scrapings of the epithelial cells and urine). Principle Of Method Real time PCR is based on the detection of the fluorescence, produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is dual-labeled DNA-probe, which specifically binds to the target region of pathogen DNA. Fluorescent signal increases due to the fluorescent dye and quencher separating by Taq DNA-polymerase exonuclease activity during amplification. PCR process consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value - Ct – is the cycle number at which the fluorescence generated within a reaction crosses the fluorescence threshold, a fluorescent signal rises significantly above the background fluorescence.