Gastrointestinal infection assay kit MOLgen

for DNAHelicobacter pyloritissue

“Molgen DNA Helicobacter pylori S1 Kit” is an assay kit for the detection of Helicobacter pylori DNA by real-time PCR. Introduction “Molgen DNA Helicobacter pylori S1 Kit” is designed to detect Helicobacter pylori DNA isolated from clinical specimens using extractionthe “Molgen Universal Extraction Kit”. Assay is based on real-time polymerase chain reaction (PCR) method with fluorescent detection of amplified product. The kit contains reagents requiredfor 48 tests, including control samples. The kit is validated for use with iQ iCycler, iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia) or equivalent. “Molgen DNA Helicobacter pylori S1 Kit” is designed for the analysis of clinical materials (stomach tissue samples). Principle Of Method Real time PCR is based on the detection of the fluorescence, produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is dual-labeled DNA-probe, which specifically binds to the target region of pathogen DNA. Fluorescent signal increases due to the fluorescent dye and quencher separating by Taq DNA-polymerase exonuclease activity during amplification. PCR process consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis. Threshold cycle value - Ct – is the cycle number at which the fluorescence generated within a reaction crosses the fluorescence threshold, a fluorescent signal rises significantly above the background fluorescence. Increased fluorescence signal is due to the use of a specific for given DNA sequence DNA hybridization probe that in the course of reaction binds with one of the DNA strands,