Staining solution reagent 05-B10005

for histology

Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 150 ml. HDPE cap. Tamper evident cap. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Reagent for evidencing cytoplasm, keratin, muscle fibers. Excellent for modified trichrome staining according to Goldner and Masson. Method 1) Sections to water 2) Iron hematoxylin, A + B solution (1 : 1), 10 minutes 3) Picric acid alcoholic solution, 3 minutes 4) Wash in distilled water 5) Ponceau Masson fuchsin, 4 minutes 6) Wash in distilled water 7) Stabilized phosphomolybdic acid solution, 10 minutes 8) Light green, 5 minutes 9) Wash in distilled water 10) Dehydrate 11) Clearing agent and mount Results Nuclei : black Cytoplasm, keratin, muscle fibers, acidophil granules of hypophysis : red Collagen, mucus : green Erythrocytes : yellow Warning and precaution The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities. Disposal Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.