Stain reagent Papanicolau EA65

for cytology

Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500 ml. HDPE cap. Tamper evident cap. Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l. HDPE cap. Tamper evident cap. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of: gynecological specimens, urine cytology, fine needle specimens, sputum and bronchial washings, to be examined by optical microscopy. Application Cytoplasmic staining solution for Papanicolaou method. For the execution of the staining method is required the use of reagents Papanicolaou Harris hematoxylin and Papanicolaou OG6. Principle A highly selective blue nuclear stain, Harris’ hematoxylin, is combined with EA65 polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from eosinophil ones. The last ingredient is OG6 solution, which stains keratinized elements. Method 1 ) Ethanol 95°, 2 minutes 2) Distilled water, 2 minutes 3) Harris Hematoxylin, 1 minute 4) Tap water, 5 minutes 5) Ethanol 95°, 15 seconds 6) OG 6, 2 minutes 7) Ethanol 95°, 15 seconds (twice) 8) EA 65, 5 minutes 9) Ethanol 95°, 15 seconds 10) Absolute Ethanol, 30 seconds (twice) 11) Xilene or Bio Clear, 2 minutes (twice) Results Nuclei : Blue-purple Cyanophil cytoplasm : Blue-green Eosinophil cytoplasm : Pink Keratinized cytoplasm : From pink to orange Warning and precaution The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases)