Staining solution reagent 05-M20001

for histology

Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500 ml. HDPE cap. Tamper evident cap. Wear, water, alcohol, and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Reagent for evidencing tissue components characterized by adjacent glycolic or aminohydroxylic groups. Principle In the PAS method, to show the neutral mucins, the periodic acid oxidizes selectively groups: 1.2 - Glycolic; primary aminic (or 1-hydroxy-2-aminic); secondary aminic (or 1hydroxy-2-alkylaminic) and 1- hydroxy-2-ketonic. During oxidating process, the links between carbon atoms in 1,2 position break and consequently aldehydic groups are formed. In the following reaction, sulphurous fuchsin in Schiff reagent changes these 2 contiguous aldehydic groups into an insoluble stained compound. Method 1) Sections to water 2) Periodic acid solution, 10 minutes 3) Wash in distilled water 4) Schiff reagent Hotchkiss McManus, 20 minutes 5) Wash in distilled water 6) Potassium metabisulfite, 2 minutes (twice) 7) Wash in distilled water, 1 minute 8) Mayer’s Hemalum, 3 minutes 9) Colour change in tap water, 5 minutes 10) Dehydrate 11) Clearing agent and mount Positive PAS substances: red magenta Nuclei: purple blue Warning and precaution The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk, and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged.