Staining solution reagent Sudan III

for histology

Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 150 ml. HDPE cap. Tamper evident cap. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Reagent for lipid staining. Method For histology 1) Sections to ethanol 95° 2) Sudan III, 10 minutes 3) Differentiate rapidly in ethanol 95° 4) Wash in distilled water 5) Mount in acqueous mount For fecal staining 1) Add few drops of Sudan III to a thin layer of fresh fecal sample on a glass slide. Neutral fats turn orange in large droplets. 2) In case of suspected malabsorption syndrome, the sample must first be acidified with two drops of acetic acid then gently heated with a small flame and then colored with Sudan III. Non-absorbed fats are strongly orange colored. 3) A malabsorption syndrome is suspected when the fecal sample is negative for the first stain (point 1) and positive for the stain described in point 2. Results Lipids : red Warning and precaution The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities. Disposal Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.