Staining solution reagent 05-M12005

for histology

Wear, water, alcohol, and solvents resistant PVC label. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Reagent used with May Grünwald for the staining of different kind of cells in blood and bone marrow smears. This staining is also used to highlight the Helicobacter pylori in histology. For the execution of the staining method is required the use of May Grünwald solution. Principle Two dyes are used one after the other: - May Grünwald solution, consisting of eosin-methylene blue, stains nuclei blue and basophil cytoplasm in pinkish red; - Giemsa solution, complex consisting of methylene blue chloride, eosin-methylene blue and azure II eosinate, improves the intensity of nuclear staining and the capacity to show selectively cellular structures. When evaluating the results, it should be remembered that the colour pattern is strongly influenced by the pH of the wash water; the intensity of the colour may vary depending on Method 1) Air dry smears 2) May Gruenwald solution 5 minutes 3) Wash in tap water 1 minutes 4) Giemsa working solution* 15 minutes 5) Wash in tap water 1-2 minutes 6) Air dry *Preparation of Giemsa working solution: dilute Giemsa solution in distilled water at ratio 1 : 10 (1 part of Giemsa solution + 9 parts of distilled water). Use within 24 hours. Results Nuclei: violet, red purple Lymphocyte cytoplasm: different blue tonalities Monocyte cytoplasm: blue grey Eosinophil granulocytes (acidophil granules): brick red – orange Basophil granulocytes (basophil granules): dark violet Neutrophil granulocytes (neutrophil granules): pink brown Erythrocytes: pink grey