Stain reagent 05-12011

for cytology

Fast staining, bright colors and excellent cellular details. The solutions are methanol-free. Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500 ml. HDPE cap. Tamper evident cap. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of: gynecological specimens, urine cytology, fine needle specimens, sputum and bronchial washings, to be examined by optical microscopy. Application Nuclear stain for Papanicolaou method. For the execution of the staining method is required the use of reagents Papanicolaou OG6. Method 1) Ethanol 95°, 2 minutes 2) Distilled water, 2 minutes 3) Harris Haematoxylin, 1 minute 4) Tap water, 5 minutes 5) Ethanol 95°, 15 seconds 6) OG 6, 2 minutes 7) Ethanol 95°, 15 seconds (twice) 8) EA 65, 5 minutes 9) Ethanol 95°, 15 seconds 10) Absolute Ethanol, 30 seconds (twice) 11) Xylene or Bio Clear, 2 minutes (twice) Results Nuclei: Blue purple Cyanophil cytoplasm: Blue green Eosinophil cytoplasm: Pink Keratinized cytoplasm: From pink to orange