Stain reagent Papanicolau OG6

for cytology

Primary container: transparent bottle in polyethylene terephthalate (PET). Useful capacity 2.5 litres. HDPE cap. Tamper evident cap. The bottle has an optimal grip. The absence of the handles reduces space for storage. The anti-dropping cap permits a precise and clean use. Secondary container: carton box. Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l. HDPE cap. Tamper evident cap. Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 500 ml. HDPE cap. Tamper evident cap. Expected aim Product for the preparation of gynaecological specimens, urine cytology, fine needle specimens, sputum, and bronchial washings, to be examined by optical microscopy. Application Cytoplasmic staining for the keratinized cells in the Papanicolaou method. For the execution of the staining method is required the use of reagents Papanicolaou Harris haematoxylin and Papanicolaou EA50. Principle A highly selective blue nuclear stain, Harris’ haematoxylin, is combined with EA50 polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from eosinophil ones. The last ingredient is OG6 solution, which stains keratinized elements. Method 1) Ethanol 95°, 2 minutes 2) Distilled water, 2 minutes 3) Harris Haematoxylin, 1 minute 4) Tap water, 5 minutes 5) Ethanol 95°, 15 seconds 6) OG 6, 2 minutes 7) Ethanol 95°, 15 seconds (twice) 8) EA 65, 5 minutes 9) Ethanol 95°, 15 seconds 10) Absolute Ethanol, 30 seconds (twice) 11) Xylene or Bio Clear, 2 minutes (twice) Results Nuclei: Blue purple Cyanophil cytoplasm: Blue green Eosinophil cytoplasm: Pink