Stain reagent 05-M12002

Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Staining method for different kind of cells in blood and bone marrow smears. For the execution of the staining method is required the use of Giemsa solution. - Two dyes are used one after the other: May Grünwald solution, consisting of eosin-methylene blue, stains nuclei blue and basophil cytoplasm in pinkish red; - Giemsa solution, complex consisting of methylene blue chloride, eosin-methylene blue and azure II eosinate, improves the intensity of nuclear staining and the capacity to show selectively cellular structures. To appreciate results always remember two factors: pH of washing waters and dilution buffer have a strong influence on final colour chart; intensity of stain may vary according to differentiation time. Method 1) Air dry smears 2) May Grünwald solution , 5 minutes 3) Wash in tap water, 1 minute 4) Giemsa ready to use solution, 15 minutes 5) Wash in tap water, 1-2 minutes 6) Air dry Results Nuclei : violet red purple Lymphocyte cytoplasm : different blue tonalities Monocyte cytoplasm : blue-grey Eosinophil granulocytes (acidophil granules) : brick red – orange Basophil granulocytes (basophil granules) : dark violet Neutrophil granulocytes (neutrophil granules) : pink-brown Erythrocytes : pink –grey Warning and precaution Over time, the product may form precipitates that do not compromise the yield/functionality/performance of the dye.