AccuPower® RT PreMix contains premixed dried components essential for cDNA synthesis such as reverse transcriptase and RNase inhibitor, allowing RT reaction to simply start by just adding the RNA, primers, and D.W. Furthermore, by using the RNase H+ of M-MLV Reverse Transcriptase, template RNA is removed after the cDNA synthesis to minimize the effect of residual template RNA in PCR reaction.
※Master Mix product is shipped in dry ice.
Features and Benefits
Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests easily simply by adding template DNA and primers
Addition of stabilizer and RNase inhibitor for high resistance to degradation
Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables
• First-strand synthesis of cDNA from RNA molecules (RT)
• Random priming reaction
• cDNA library construction
• Probe labeling
• mRNA 5’-end mapping by primer extension analysis
• Real-time qPCR
DNase activity No
RNase activity No
Fragment size Up to 9 kb
AccuPowee RT PreMix is an easy to resuspend, vacuum-dried premix for cDNA synthesis that consists of M-MLV(Mo/oneyMurine Leukemia Virus) reverse transcrip-tase, RNase inhibitor, dNIPs, reaction buffer, tracking dye, and patented stabilizer. All of the key components are premixed at optimal concentrations. Simply add template RNA primers and D.W. for cONA synthesis reaction.