DNA polymerase reagent kit / for research / for DNA
E-31 series

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DNA polymerase reagent kit / for research / for DNA DNA polymerase reagent kit / for research / for DNA - E-31 series
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Characteristics

  • Type:

    DNA polymerase

  • Applications:

    for research

  • Sample type:

    for DNA

Description

Top DNA polymerase contains a thermostable DNA polymerase having improved polymerase activity by using the recombinant DNA technology on a thermophilic polymerase gene.

※This product is shipped in dry ice.

Features and Benefits
Optimized buffer delivery
Guaranteed stable response with Top optimized buffer for DNA polymerase

Efficiency & Sensitivity
High efficiency and sensitivity for DNA amplification using PCR

Reproducibility
Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system

Application
Real-time quantification of DNA and cDNA targets using dsDNA binding dye
Gene expression profiling
Microbial & viral pathogen detection

Specifications
5' to 3' exonuclease activity No
3' to 5' exonuclease activity No
3' – A overhang Yes
Fragment size Up to 10 kb

• 10X reaction buffer with (or without) MgCl2: Tris (pH 9.0), 15 mM MgCl2, etc

• 1X dilution buffer: 50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0

• 10 mM dNTPs mix: 2.5 mM of each dNTP

▶ Concentration
500 Units (5 U/μl)

▶ Storage conditions
50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0

▶ Storage temperature
-20°C

▶ Unit Definition
One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.

PRODUCT AND COMPANY INFOMATION
Product Name : Top DNA polymerase
Product Number : E-3100, E-3100-1, E-3100-2, E-3100-3
Product Use : For research use only and are not intended for human or animal diagnostics,
therapeutic , or other clinical uses, unless otherwise stated.