This product contains a reverse transcriptase removed with RNase activity for an effective synthesis of long size cDNAs.
※This product is shipped in dry ice.
Features and Benefits
Long cDNA Synthesis
Effective synthesis of cDNA up to 12.5 kb by removing the activity of RNase H
Comprehensive compatible temperature for effective cDNA synthesis according to customer's experimental conditions from 42 ℃ to 70 ℃, with enzymes developed based on RocketScript™ Reverse Transcriptase products
Excellent amplification sensitivity even with a trace amount of 1 pg RNA
Reproducible results with uniform quality products supplied for each batch under the ISO 9001 quality system
First-strand synthesis of cDNA from RNA molecules (reverse transcription)
Random priming reactions
mRNA 5’-end mapping by primer extension analysis
DNase activity No
RNase acitivity No
RNase H activity No
Fragment size ~12.5 kb
• 5X Reaction Buffer: Tris-HCl, KCl, MgCl2, Stabilizers (pH8.3)
• 100 mM DTT
• 10 mM dNTPs mix: 2.5 mM of each dNTP
10,000 Unit (200 U/μl)
50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, Stabilizers.
One unit is defined as the amount of enzyme required to incorporate 1 nmole of deoxynucleotide into acid-precipitable material in 10 min at 37℃ using poly A, oligo dT as template primer