AccuPower® PyroHotStart Taq PCR PreMix is applied with Bioneer's enzyme-mediated HotStart patent technology to enhance response specificity and PCR amplification. Taq DNA polymerase reduces non-specific reactions like the mis-priming and primer dimer formation that may occur at a low temperature. In addition, the components necessary for PCR such as DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run.
Features and Benefits
Minimized generation of non-specific amplification products, maximized PCR reaction efficiency, and effective amplification of template DNA present in a small amount through the applicaiton of PyroHotStart technology
Enhanced stability for maintaining stable enzymatic activities for a long-term storage by including stabilizer and dried in the PCR reaction mixture
Simplified procedure with all necessary components and DNA polymerases added in the PCR tubes to perform the PCR reaction immediately just by adding the template DNA, primer sets, and D.W., along with tracking dye and sedimentation agents for electrophoresis without the need of sample loading buffer
Reproducible experimental results provided through mass production of one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product
(a) Inhibition of PCR reaction in the zero cycle
After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.