AccuPower® Gold Multiplex PCR PreMix can generate amplified PCR products of 20 target genes simultaneously with only one reaction and a tube. By applying Bioneer’s patent technology of enzyme-mediated HotStart, chances of non-pecific reactions that can occur when DNA polymerase reacts at low temperatures such as mis-priming and primer-dimer are reduced. This product is compatible with genotyping assay and genetic diagnosis that utilizes multiplex PCR technology.
Features and Benefits
Generation of 20 multiplexed amplification products in a single tube with only one reaction
Minimized non-specific amplications by application of PyroHotStart technology which includes pyrophosphatase to maximize the efficiency by eliminating the PCR inhibitor pyrophosphates produced every cycle.
Shortened calculation time of PCR condition using PyroHotstart technology to easily amplify multiple templates from 1 to 20, with anyone capable of initiating the PCR process just by adding the template DNA and their corrosponding primer sets
Enhanced stability allowing long-term storage by including the stabilizer and drying in the PCR reaction mixture
Reproducible experimental results by mass production in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality products
(a) Inhibition of PCR reaction in the zero cycle
After the preparation of the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.