DNA polymerase reagent kit AccuPower® HotStart

for PCRprotein

AccuPower® HotStart Pfu PCR PreMix is applied with Bioneer's enzyme-mediated HotStart patent technology to enhance response specificity and PCR amplification, along with reduction of non-specific reactions such as mis-priming and primer-dimer that may occure during Pfu DNA polymerase reaction at low temperatures. In addition, the proof-reading (3 '→ 5' exonuclease) function of Pfu DNA polymerase reduces the error in DNA amplification reaction, making it suitable for cloning experiments for protein production. Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer. Furthermore, stabilizers are added to keep the activity stable for one month when stored at room temperature (25°C) and for two years when stored in a freezer. ※PreMix product is shipped at room temperature. The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature. Features and Benefits Fidelity High fidelity (error rate= 1.9X10-6) with minimized mutation during DNA amplification Specificity Selection of specific sequences by applying PyroHotStart technology to the proof-reading function of Pfu DNA polymerase, allowing the amplified products to be used in experiments that require accurate sequences such as cloning and mutagenesis after simple PCR purification processes User-friendly Simplified procedure by requiring only the addition of template DNA, primer sets, and D.W., with all necessary components and DNA polymerases included and mixed in each of the tubes packaged with sufficient amount for 1 PCR run,