Solution reagent kit pSpark® I

for PCRfor nucleic acids

For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use Product Overview: pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency. The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector. Advantages & Features Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by our Quality 100% Guarantee.