For highly Efficient, Accurate & Robust general Cloning from PCR High Fidelity fragments, without toxic genes use
pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency.
The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.
Advantages & Features
Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes.
Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
High stability: eliminates cloning bias or pitfalls.
Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert.
Compatible with blue/white screening.
Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
Eliminates positive selection vector.
High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
Risk-free: product covered by our Quality 100% Guarantee.