For highly Efficient, Accurate & Easy Cloning with Ampicillin and Kanamycin resistance
pSpark® III is a highly efficient, accurate and easy-to-use DNA Cloning System that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.
Advantages & Features
Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
Powerful: obtain 5-fold more positive colonies using 10x less DNA insert.
Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes.
High stability: eliminates cloning bias or pitfalls.
Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
Eliminates positive selection vector.
Risk-free: product covered by our Quality 100% Guarantee.
Includes for 20 rxn:
– 20 µL pSpark® III (20 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 200 µL T4 DNA Ligase Buffer (5x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)