For highly Efficient, Stable & Powerful Cloning under transcription-free conditions
pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation.
Advantages & Features
Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%).
High stability: eliminates cloning bias or pitfalls.
Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers.
Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
Cost avoidance: removes expensive primer phosphorylation use.
Eliminates positive selection vector.
Risk-free: product covered by our Quality 100% Guarantee.