DNA ligase reagent kit pSpark® IV
for PCRfor nucleic acids

DNA ligase reagent kit
DNA ligase reagent kit
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DNA ligase
for PCR, for nucleic acids


For highly Efficient, Stable & Powerful Cloning under transcription-free conditions Product Overview: pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation. Advantages & Features Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Transcription-free. Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%). High stability: eliminates cloning bias or pitfalls. Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating. Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert. Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Cost avoidance: removes expensive primer phosphorylation use. Eliminates positive selection vector. Risk-free: product covered by our Quality 100% Guarantee.


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