For highly Efficient, Accurate & Easy Cloning of PCR fragments with EcoRI and NotI flanking the insertion site
pSpark® Done is a highly efficient, accurate and easy-to-use DNA Cloning system designed for cloning of blunt ended DNA with very high efficiency. The MCS of the pSpark® Done vector incorporates sequences on either side of the insert that are recognized by the restriction enzymes NotI and EcoRI. This allows the insert DNA to be removed with a single restriction digest using either of these enzymes
Advantages & Features
Optimized: recognition sites for Not I and EcoR I at either side for the insertion of the cloning point.
Flexible: allows removing the desired insert DNA with other restriction digestion.
Unprecedented efficiency: more than 2,500 positive colonies expected under optimal conditions.
Easy-to-use: eliminate screening of recombinants due to its its minumum background (lower than 1%).
Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
High stability: eliminates cloning bias or pitfalls.
Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
Eliminates positive selection vector.
Cost avoidance: removes expensive primer phosphorylation use.
Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
Risk-free: product covered by our Quality 100% Guarantee.