Solution reagent kit CVX5α™

for genotypingcalcium

Versatile, Convenient & Cost-effective solution for routine Subcloning Procedures Product Overview: CVX5α™ Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane. CVX5α™ Genotype: F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ). Advantages & Features Versatile: proven performance for high-efficiency transformation in a wide variety of applications. Convenient: ideal for routine. Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. Cost avoidance: dry ice free of charge. Specifications CVX5α™ Genotype: F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ). Includes Includes for 40 rxn: – 2,000 µL CVX5α™ (1 x 107 CFU/µg) – 10 µL pUC18 Transformation Control Plasmid (10 ng/µL) – 50 mL SOC Medium – Dry ice Applications Routine cloning and subcloning of genes into plasmid vectors. Quality Control Each lot of competent cells is tested to verify transformation efficiencies using 100 pg pUC18 supercoiled DNA and the recommended protocol. Under these conditions, transformation efficiency will be ≥ 1 x 107 cfu/μg pUC18. Transformation efficiency is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid (3 kb) into a given volume of competent cells.