For highly Efficient, Accurate & Easy Cloning with pBR322 and Transcription-free conditions
pSpark® V is a highly efficient, accurate and easy-to-use DNA cloning system developed with low copy number, as a help for cloning of inserts with the highers kb. This low copy variant is also transcription-free, for the most demanding cloning tasks. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region has been truncated).
Advantages & Features
Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%).
High stability: removes cloning bias or pitfalls.
Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
Optimized: truncated alpha-peptide coding region.
Cost avoidance: avoids expensive primer phosphorylation use.
Eliminates positive selection vector.
Risk-free: product covered by our Quality 100% Guarantee.
Includes for 20 rxn:
– 20 µL pSpark® V (20 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 200 µL T4 DNA Ligase Buffer (5x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)