For highly Efficient, Accurate & Easy general Cloning with classical MCS, without the use of Toxic Genes
pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases.
The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.
Advantages & Features
Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
Great efficiency: over hundreds positive colonies with few nanograms of insert.
High stability: eliminates cloning bias or pitfalls.
Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
Powerful: clone from less than 1 ng/kb to up to 14 kb; obtain 4-fold more positive colonies using 3-fold less DNA insert.
Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes.
Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers.
Flexible: ligation time from 10 minutes to overnight.
Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed.
Eliminates positive selection vector.
Risk-free: product covered by our Quality 100% Guarantee.