IgG reagent 0855226

enzymeantibodyfor research

Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG Fc and buffer salts. Application Notes Peroxidase-conjugated goat IgG fraction to human IgG Fc is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals. Specifications Concentration - Total Protein: 39.0 mg/mL; Titer: 1:200;000 Conjugate - HRP Grade - Molecular Grade Immunogen - Human IgG (Fc) fragment Pack Size - 2 mL Preparation Method - Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. Purity - Purified Solubility - Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material. Species Reactivity - Human