IgG reagent 0855641

antibodyenzymefor research

Product is goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts. Application Notes Goat affinity purified antibody to rabbit IgG (Whole molecule) is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques, for conjugation with enzymes, radiolabels, fluorochromes or other markers, attachment to solid supports for use as an immunoadsorbant and used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). Specifications Concentration - Total Protein: 1.06 mg/mL; Active Antibody: 1.0 mg/mL Conjugate - Unconjugated Formulation Details - Purified Grade - Molecular Grade Host - Goat Immunogen - Rabbit IgG Pack Size - 2 mg Personal Protective Equipment - Eyeshields, Gloves, respirator filter Preparation Method - The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. Purity - Affinity Purified Solubility - Soluble in 1 mL of 0.135 M sodium chloride. Species Reactivity - Rabbit Specificity - It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG;