IgG reagent 0855391

enzymeantibodyfor research

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to guinea pig IgG (whole molecule) and buffer salts. Application Notes This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. Specifications Concentration - Total Protein: 15.9 mg/mL Titer: 1:176;000 Grade - Molecular Grade Pack Size - 2 mL Preparation Method - Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. No preservative has been added. Purity - Purified Solubility - Soluble in water Species Reactivity - Guinea Pig Specificity - It shows reactivity to guinea pig IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.