Protein reagent 0855385

reagent mediumfor researchtissue

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to guinea pig complement C3 and buffer salts. Application Notes Fluorescein-Conjugated Goat IgG Fraction to Guinea Pig Complement C3 is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. This product is especially useful in anti-complement immunofluorescent (ACIF) assays. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Specifications Concentration - Total Protein: 16.3 mg/mL Conjugate - FITC Grade - Molecular Grade Host - Goat Immunogen - Guinea Pig Complement (C3) Pack Size - 2 mL Preparation Method - Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. Purity - Purified Solubility - Soluble in water Species Reactivity - Guinea Pig Specificity - This product shows reactivity to guinea pig complement C3; cross-reactivity to other species may exist. Monospecific for the third component; of guinea pig complement (C3). Typical Working Concentration - Suggested working dilution: Anti-complement immunofluorescent (ACIF) assays: 1:15