IgG reagent 0855167

antibodyfor ELISAtissue

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human complement C3 and buffer salts. Application Notes Fluorescein-conjugated Goat IgG Fraction to Human Complement C3 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). It is also used for Immunohistochemistry techniques at a dilution of 1:50. Specifications Conjugate - FITC Grade - Molecular Grade Immunogen - Human C3 purified from serum Pack Size - 2 mL Preparation Method - Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. Solubility - Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. Species Reactivity - Human Specificity - This product shows reactivity to human complement C3; cross-reactivity to other species may exist. Antibody activity to complement components C4-C9 or serum proteins is not present. Typical Working Concentration - Dilution Recommendations: 1:100 - 1:400 for Tissue Staining; 1:1,000 - 1:4,000 for ELISA/FIA