Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse complement C3 and buffer salts.
Product Description
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse complement C3 and buffer salts.
Application Notes
Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Specifications
Application Notes - Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration - Total Protein: 8.3 mg/mL; Antibody Protein: 4 mg/mL
Conjugate - FITC
Grade - Molecular Grade
Host - Goat
Pack Size - 2 mL
Preparation Method - Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity - Purified
Solubility - Soluble in water