IgG reagent 0855168

antibodyfor researchimmunohistochemistry

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human complement C4 and buffer salts. Application Notes FITC-Conjugated Goat IgG Fraction to Anti-Human Complement C4 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use Specifications Concentration - Total Protein: 7.50 mg/mL Conjugate - FITC Grade - Molecular Grade Host - Goat Pack Size - 2 mL Preparation Method - Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. Solubility - Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. Specificity - This product shows reactivity to human complement C4; cross-reactivity to other species may exist. Antibody activity to complement components C3; C6-C9 or serum proteins is not present. Typical Working Concentration - Dilution reccomendations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA