IgG reagent 0855676

serumgoat serumantiserum

Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. IgG fraction of antiserum is essentially free of other goat serum proteins. Product Description Peroxidase-conjugated goat igg fraction to rabbit IgG (whole molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Application Notes Peroxidase-conjugated goat IgG fraction to rabbit IgG (whole molecule) product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. Specifications Concentration - Total Protein: 6.9 mg/mL Titer: 1:180;000 Conjugate - HRP Grade - Molecular Grade Host - Goat Pack Size - 2 mL Personal Protective Equipment - Eyeshields, Gloves, half-mask respirator , multi-purpose combination respirator cartridge Preparation Method - Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation.