Membrane ultrafilter Vivaspin® 500

for liquidsfor protein solutionsfor serum

Product overview
Vivaspin® 500 centrifugal concentrators are designed for rapid concentration of protein and nanoparticle samples with starting volumes up to 500 µl. The devices are intended for use in micro-centrifuges with fixed-angle rotors that accept 2.2 ml (11 mm) conical bottom tubes. The patented vertical membrane geometry and PES membranes reduce filter fouling and shorten spin times while the built-in dead-stop pocket maximizes concentrate recovery.

Downloads available
Data Sheet; Technical Data and Operating Instructions; CAP Validation Procedures; Urine Protein Concentration with Vivaproducts Concentrators.

Applications

• Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
• Concentration of cerebrospinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
• Protein removal and sample preparation from serum and cell/tissue lysates.
• Buffer exchange for proteomic peptide samples.
• Sample preparation for mass spectrometry and downstream analytical workflows.

Performance (selected)
Rotor — fixed angle
Centrifugal force — up to 12,000 x g
Start volume — up to 500 µl
Aprotinin 0.25 mg/ml (6,500 MW) — 3,000 MWCO PES — Time to concentrate up to 30x: 30 min — Concentrate recovery: 96%
BSA 1.0 mg/ml (66,000 MW) — 5,000 MWCO PES — Time: 15 min — Recovery: 96%
BSA 1.0 mg/ml — 10,000 MWCO PES — Time: 5 min — Recovery: 96%
BSA 1.0 mg/ml — 30,000 MWCO PES — Time: 5 min — Recovery: 95%
IgG 0.25 mg/ml (160,000 MW) — 30,000 MWCO PES — Time: 10 min — Recovery: 96%
IgG 0.25 mg/ml — 50,000 MWCO PES — Time: 10 min — Recovery: 96%
IgG 0.25 mg/ml — 100,000 MWCO PES — Time: 10 min — Recovery: 96%

Technical specifications (summary)
Concentrator capacity: designed for micro-volumes up to 500 µl; do not use in swing-bucket rotors. Fixed-angle rotor cavity fits 2.2 ml (11 mm) conical bottom tubes; minimum rotor angle 40°.
Dimensions: total length 50 mm; width 11 mm; active membrane area 0.5 cm².
Hold-up volume (membrane and support) <5 µl; dead-stop volume 5 µl.
Materials: body and cap — polycarbonate; filtrate vessel — polypropylene; membrane — polyethersulfone (PES).

Equipment required
Microcentrifuge with fixed-angle rotor (minimum rotor angle 40°) to accept 2.2 ml (11 mm) conical tubes. Maximum speed up to 12,000 x g. Pipettes: fixed or variable volume; recommended tips: thin gel-loader type for accurate concentrate recovery.

Ordering (examples)
Available membrane options: MWCO PES from 3,000 to 1,000,000 and 0.2 µm PES. Pack sizes: 25 or 100 per pack. Pricing varies by MWCO and pack size; consult product listing for SKU details.

Features / technical specifications

• Model: Vivaspin® 500 — concentrator for micro-volumes up to 500 µl.
• Maximum concentration factor: up to 100x (dependent on MWCO and sample).
• Compatible rotor: fixed-angle only (not for swing-bucket rotors); fits 2.2 ml (11 mm) conical bottom tubes; minimum rotor angle 40°.
• Maximum centrifugal force: 12,000 x g.
• Active membrane area: 0.5 cm².
• Hold-up volume <5 µl; dead-stop volume 5 µl.
• Construction materials: polycarbonate (body and cap), polypropylene (filtrate vessel), PES membrane.
• Membrane/MWCO options: 0.2 µm and 3,000–1,000,000 MWCO.
• Pack sizes: 25 or 100 per pack.
• Typical concentrate recovery: ~95–96% (dependent on MWCO and analyte).