Membrane ultrafilter Vivaspin® 20

for liquidsfor protein solutionsfor serum

Product overview
Vivaspin® 20 centrifugal devices enable rapid concentration of protein and nanoparticle samples with starting volumes up to 20 ml. They are compatible with rotors that accept 50 ml centrifuge tubes or can be operated using controlled air pressure. The patented twin-vertical PES membrane design reduces filter fouling and delivers fast spin times, while the built-in dead-stop pocket maximizes concentrate recovery. Selected Vivaspin® 20 variants are IVD-capable and have been used to concentrate urine prior to electrophoresis for diagnosis of multiple myeloma, light-chain amyloidosis and related hematological disorders. Sebia and Helena Labs recommend Vivaspin® 20 devices for preparing urine samples for their capillary electrophoresis systems.

Key features
Up to 20 ml starting volume
Concentrate up to 400x

Applications

• Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis.
• Concentration of cerebrospinal fluid prior to electrophoresis for diagnosis of meningitis and multiple sclerosis.
• Protein removal from serum and cell or tissue lysates.
• Buffer exchange for proteomic peptide samples.
• Sample preparation for mass spectrometry.

Performance (selected results: time to concentrate up to 30x at 20°C and solute recovery %)
Mode / Test / Membrane / Centrifuge/Pressure / Start volume / Min. / Recovery%
Cytochrome c 0.25 mg/ml (12,400 MW): 3,000 MWCO PES — Swing bucket 3,000 g — Start 20 ml — Min. 110 — Rec. 97%
BSA 1.0 mg/ml (66,000 MW): 5,000 MWCO PES — 3,000 g / 6,000 g / Pressure 4 bar — Start vol 20/14/10 ml — Min. 23 / 29 / 50 — Rec. 99% / 99% / 98%
BSA (10,000 MWCO PES): Min. 16 / 17 / 32 — Rec. 98% / 98% / 97%
IgG 0.25 mg/ml (160,000 MW): 30,000 MWCO PES: Min. 27 / 20 / 46 — Rec. 97% / 95% / 94% (various conditions); 50,000 MWCO and 100,000 MWCO results also provided with comparable recovery percentages.
Latex beads (0.055 µm): 300,000 MWCO PES — Min. 20 — Rec. 99%
Latex beads (0.24 µm): 1,000,000 MWCO PES — Min. 4 — Rec. 99%
Yeast 1.0 mg/ml (S. cerevisiae): 0.2 µm PES — Min. 15 / 5 / 20 / 2 — Rec. 95% across tested modes

Technical Specifications

• Concentrator capacity: Swing Bucket Rotor 20 ml; 25° Fixed Angle Rotor 14 ml; With Pressure Cap 15 ml
• Dimensions: Total length 116 mm; Width 30 mm; Active membrane area 6.0 cm²
• Hold-up volume (membrane): <20 µl; Dead stop volume: 50 µl
• Carrier required: Insert diameter 29 mm; Conical bottom tube: 50 ml
• Maximum pressure: 5 bar
• Materials of construction: Body and filtrate vessel: Polycarbonate; Concentrator cap: Polypropylene; Membranes: Polyethersulfone (PES); Pressure head: Acetal & Aluminum
• Maximum speed (recommended): up to 4,000 g (swing bucket) or up to 6,000 g (fixed angle) depending on rotor and membrane MWCO; devices with membrane MWCO ≥ 100 kDa should be processed at lower g forces (see data sheet).
• Accessories available: Air Pressure Controller (APC), pressure heads, pneumatic tubing, connectors, diafiltration cups and replacement seals.