Membrane ultrafilter Vivaspin® 2

for liquidsfor protein solutionsfor serum

Overview Vivaspin® 2 centrifugal devices enable rapid concentration of protein and nanoparticle samples up to 2 ml (fixed-angle rotor) or 3 ml (swing-bucket rotor). Available with PES, CTA or Hydrosart® membranes, the patented vertical membrane design reduces fouling and a built-in dead-stop pocket maximizes concentrate recovery. Reverse spinning allows simplified recovery into the upper cap for clinical and preparative workflows.

Key features

• Starting volume: up to 2 ml (fixed-angle) / 3 ml (swing-bucket)
• Concentration factor: up to 250×
• Membrane options: PES, CTA, Hydrosart® (HY)
• Patented vertical membrane and dead-stop pocket for high recovery
• Compatible with rotors accepting 15 ml (17 mm) conical tubes

Applications

• Concentration of urine prior to electrophoresis for diagnosis of multiple myeloma and amyloidosis
• Concentration of cerebrospinal fluid before electrophoresis for diagnosis of meningitis and multiple sclerosis
• Protein removal from serum and cell/tissue lysates
• Buffer exchange for proteomic peptide samples
• Sample preparation for mass spectrometry

Downloads (document titles)

• Data Sheet
• Technical Data and Operating Instructions
• Urine Protein Concentration Calculator App (reference)
• CAP Validation Procedures (Performing the test)
• Urine Protein Concentration with Vivaproducts Concentrators

Performance (time to concentrate / concentrate recovery at 20°C) Table header — Time to concentrate up to 30× [min.] at 20°C — Concentrate Recovery %
5,000 x g (centrifugal force)
Insulin chain A 0.1 mg/ml (2,535 MW): 2,000 MWCO HY — 35 min — 95%
Aprotinin 0.25 mg/ml (6,500 MW): 3,000 MWCO PES — 50 min — 96%
BSA 1.0 mg/ml (66,000 MW): 5,000 MWCO PES — 12 min — 98%
BSA 1.0 mg/ml (66,000 MW): 5,000 MWCO CTA — 50 min — 96%
BSA 1.0 mg/ml (66,000 MW): 10,000 MWCO PES — 8 min — 98%
BSA 1.0 mg/ml (66,000 MW): 10,000 MWCO CTA — 10 min — 96%
BSA 1.0 mg/ml (66,000 MW): 10,000 MWCO HY — 12 min — 98%
BSA 1.0 mg/ml (66,000 MW): 20,000 MWCO CTA — 5 min — 96%
BSA 1.0 mg/ml (66,000 MW): 30,000 MWCO PES — 8 min — 97%
BSA 1.0 mg/ml (66,000 MW): 30,000 MWCO HY — 5 min — 97%
IgG 0.25 mg/ml (160,000 MW): 20,000 MWCO CTA — 6 min — 97%
IgG 0.25 mg/ml (160,000 MW): 30,000 MWCO PES — 10 min — 96%
IgG 0.25 mg/ml (160,000 MW): 50,000 MWCO PES — 10 min — 95%
IgG 0.25 mg/ml (160,000 MW): 100,000 MWCO PES — 8 min — 95%

Technical Specifications Concentrator Capacity — Swing Bucket Rotor: 3 ml — Fixed Angle Rotor: 2 ml
Dimensions — Total Length: 126 mm — Width: 17 mm — Active Membrane Area: 1.2 cm²
Hold-up volume (membrane and support): <10 µl — Dead stop volume: 8 µl
Materials of Construction — Body: Polycarbonate — Filtrate Vessel: Polycarbonate — Concentrator Cap: Polycarbonate — Membrane: Polyethersulfone (PES), Cellulose Triacetate (CTA), Hydrosart®

Equipment Required Centrifuge — Rotor Type: Swing bucket or fixed angle — Rotor cavity: to fit 15 ml (17 mm) conical bottom tubes — Minimum rotor angle for fixed angle: 25° — Maximum speed: swing-bucket up to 4,000 g; fixed-angle up to 8,000 g
Concentrate Recovery — Pipette type: fixed or variable volume — Recommended tip: thin gel loader type

Ordering notes (selected variant examples) Example pack / membrane / pore-size combinations and indicative pack sizes: 2,000 MWCO Hydrosart® (25 / 100), 3,000 MWCO PES (25 / 100), 5,000 MWCO PES (25 / 100), 10,000 MWCO PES (25 / 100), 30,000 MWCO PES (25 / 100), 50,000 MWCO PES (25 / 100), 100,000 MWCO PES (25 / 100), CTA and Hydrosart® variants. Prices vary by SKU and pack size.

Characteristics / technical specifications

• Model: Vivaspin® 2 — intended for sample volumes up to 2 ml (fixed-angle) or 3 ml (swing-bucket)
• Maximum concentration factor: up to 250×
• Membrane options: Polyethersulfone (PES), Cellulose Triacetate (CTA), Hydrosart® (HY)
• Active membrane area: 1.2 cm²
• Total length: 126 mm; width: 17 mm
• Dead stop volume: 8 µl; hold-up volume (membrane and support): <10 µl
• Materials: body, filtrate vessel and cap — polycarbonate
• Compatible with rotors that fit 15 ml (17 mm) conical bottom tubes; swing-bucket and fixed-angle rotors supported
• Maximum recommended speeds: swing-bucket up to 4,000 g; fixed-angle up to 8,000 g
• Recommended pipette tip for recovery: thin gel loader type